Cucumber Necrosis Virus Is a Member of the Tombusvirus Group
نویسندگان
چکیده
The electrophoretic patterns of dsRNA species isolated from cucumber plants infected with cucumber necrosis virus (CNV) and from Nicotiana clevelandii plants infected with tomato bushy stunt virus (TBSV) were almost identical and differed greatly from the electrophoretic patterns of dsRNA species isolated from tobacco plants infected with tobacco necrosis virus (TNV). Hybridization with cloned cDNA of CNV showed that there was extensive homology between the nucleic acids of CNV and TBSV but not between those of CNV and TNV. Gel diffusion tests and ELISAs showed no serological relationship between CNV and five tombusviruses. TBSVinfected N. clevelandii plants showed some cross-protection against infection by CNV but not against infection by carnation ringspot virus. CNV therefore resembles TBSV more than TNV and should be classified as a tombusvirus.
منابع مشابه
Atomic structure of Cucumber necrosis virus and the role of the capsid in vector transmission.
Cucumber Necrosis Virus (CNV) is a member of the genus Tombusvirus and has a monopartite positive-sense RNA genome packaged in a T=3 icosahedral particle. CNV is transmitted in nature via zoospores of the fungus Olpidium bornovanus. CNV undergoes a conformational change upon binding to the zoospore that is required for transmission, and specific polysaccharides on the zoospore surface have been...
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Cucumber necrosis virus (CNV) is a member of the genus Tombusvirus and has a monopartite positive-sense RNA genome. CNV is transmitted in nature via zoospores of the fungus Olpidium bornovanus As with other members of the Tombusvirus genus, the CNV capsid swells when exposed to alkaline pH and EDTA. We previously demonstrated that a P73G mutation blocks the virus from zoospore transmission whil...
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